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) An Hfr strain (arg+, bio+, leu+) is mated to an F- strain (arg-, bio-, leu-). Assume that this Hfr strain transfers arg+ last. You grow the exconjugants on arg- media, supplemented with leu+ and bio+. You recover 423 arg+ exconjugants. These are tested individually for bio and leu and you find the following distribution of genotypes:
arg+, bio+, leu+ 360
arg+, bio+, leu- 9
arg+, bio-, leu+
0 arg+, bio-, leu- 54
a) What is the gene order (show your reasoning)? b) What are the frequencies of recombination between each pair of genes?
Why would you not use G-25 to separate ferricyanide (329 Da), bovine cytochrome c (12, 000 Da) and bovine hemoglobin (64,000 Da)? From the following list of resins, choose one that would be appropriate for this purpose, and briefly explain your choice.
Resin - Fractionation Range for Proteins (Da)
Bio-Gel P-6 - 1000-6000
Bio-Gel P-30 - 2500-40,000
Bio-Gel P-100 - 5000-100,000
Bio-Gel P-200 - 30,000-200,000