GENE 540 Midterm: Epistasis Activity
Document Summary
Epistasis: the interaction of genes that are not alleles, in particular the suppression of the effect of one such gene by another. The image on the right is correct: this is because. Cdc2+cycb is needed to drive cells into m-phase, so if this is taken away, then you get a long arrested g2; and then you have wee1 affecting the cycb+cdc2. Wee1 is a dual specificity kinase it puts an inhibitory phosphate on a tyrosine residue of cdc2; it is a dual specificity kinase, which means it can phosphorylate both tyrosine and serine/threonine. Cdc25 is an phosphatase that removes the phosphate from cdc2 results in an activation of cdc2 kinase activity (mpf) and leads to the entry into m-phase. The trigger that sends g2 to m-phase is the removal of the y15 (tyrosine-15) phosphate by cdc25. Threonine (t161) needs be phosphorylated for it to be active; cak (cdk activating kinase) puts a phosphate on t161.
