GENE 540 Study Guide - Midterm Guide: Dna Replication Factor Cdt1, Cdc6, Helicase
Document Summary
Dna replication in yeast dependent kinase compared to the virus. This process is controlled by cyclins and cyclin. There are more proteins involved in yeast: cyclin dependent kinase regulates it, depending on what cyclin binds to it, that will indicate if the replication process will be active or not mitosis and early g1, when cdk. Step 1 : during exit from activity is low, the mcm loading factors orc, cdc6, and cdt1 load the replicative helicase, the mcm complex, onto dna at replication origins. to the orc when they are not. Cdc6 and cdt1 can only bind phosphorylated; once cdc6 and. Cdt1 proteins have bound to orc, they can then recruit the helicase. Cdks and ddk marks the onset of s. Step 2 : activation of s phase phase. Mcm helicase, sld2, and sld3 (depicted as green phosphorylation. Cdc45-sld3 and gins complexes onto sites of replication initiation. Loading of these activators leads mcm helicases to unwind dna.
