BIO_SC 2300 Study Guide - Midterm Guide: Scanning Electron Microscope, Transmission Electron Microscopy, Confocal Microscopy
8 May 2019
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Document Summary
At the intersection of genetics, biochemistry, and cytology. Genetics-- determines everything about a cell, ie proteins produced. Cells vary vastly in shape and size. Appearance, behavior, and function of cells are dictated by proteins. Study of cellular structure, what you see in a microscope. Came about with the advent of microscopy. As more sophisticated methods of microscopy were developed, greater resolution and magnification led to rapid discoveries. Uses a beam of electrons instead of light and magnetic coils to focus the beam. Tem (transmission electron microscopy) allows for high resolution imaging of thin sections. Used on fixed, dried, and stained samples. Specimens are coated with a film of metal (platinum or gold) that causes the electrons to scatter. Emitted electrons are picked up by a detector. A computer is used to create the image. Incredible resolution, but not as good magnification as tem. Useful for looking at specific components of a cell ie plasma membrane, nucleus.
