BIOL-K - Biology BIOL-K 325 Final: EXAM 3 STUDY GUIDE
14 Apr 2016
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Move volume = final volume / (df -1) Diluent volume = final volume move volume. Total mixing volume = diluent volume + move volume. Make a 7-point 1:3 standard curve, staring neat, such that you can pipete duplicates of 50 ul per well: calculate the minimum diluent volume per step: 50 ul per well * 2 for duplicates = 100 ul minimum. Add extra volume to compensate for pipeing error, for example, 20 ul, which brings our desired diluent. Volume to 120 ul: calculate move volume: move volume = 120 ul / (3-1) = 60 ul, calculate total mixing volume: total mixing volume = 120 ul + 60 ul , graph. Use to calculate concentraion at speciic absorbance. Don"t count values outside of standard curve: bca (570): does not react with sds, but takes longer, bsa: bradford assay. Sds-page: move towards (+) end, sds denatures proteins, disrupts charge, dissociates mulimeric proteins, binding gives (-) charge to proteins.
