ECON-E 201 Study Guide - Quiz Guide: Lac Operon, Plasmid, Dicer
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ECON-E 201 Full Course Notes
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Explain the features of the bacteria and how this system works to make dsrna from the plasmid. Draw a diagram if it would help. (4 points: pl4440 is the plasmid name, ht115(de3) is the bacteria, the bacteria have a genomic insertion that puts the t7 polymerase under the control of the lac operator. When iptg is present, the bacteria make t7 polymerase, which then binds to the t7 promoter in the plasmid. The plasmid carries a gene of interest between t7 promoter sequences on both sides. The t7 polymerase transcribes ssrna from both directions in the plasmid.
