NSCI 1403 Study Guide - Final Guide: Survivorship Curve, Herbivore, Pyrimidine
9 Jul 2016
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Transgenic organism: selectively choosing genes of interest in order to make beneficial transgenic organism. Goals: snipping out gene of interest & using a vector to transfer gene from one place to another. Step 1: using restriction enzyme we isolate the gene of interest. Step 2: vector is used for gene transfer. Make a nick in the plasmid (plasmid is a type of vector) Step 3: make bacteria walls more permeable so they soak up recombinant plasmid. Plasmids as vectors: small dna molecule within a cell physically separated from chromosomal dna and can replicate independently. Has 1 or more restriction enzyme recognition sequence. Ex vivo: extract target cells from patient, genetic variation in lab, and then insert them back into the patient. In vivo: genes are inserted directly into the patient, targeted to appropriate cells: dna fingerprinting. Dna has phosphate groups that are negatively charged. Through an electric field they can be separated, making breaks in places depending on dna.
