PCB 3063 Study Guide - Summer 2018, Comprehensive Midterm Notes - Dna, Protein, Rna
PCB 3063
MIDTERM EXAM
STUDY GUIDE
Fall 2018
De novo
- starts from the beginning = doesn't require a primer
- ex: RNA synthesis can be "de novo"; DNA synthesis can't be
Taylor, Woods, Hughes experiment
- pulse chase style on beans
- used ³H thymidine
Procedure:
- grow beans in radioactive ³H thymidine
- switch to cold (non radioactive) thymidine
- stop cells at metaphase
- squash cells and dip them in photographic emulsion
Findings:
- inactivated X replicated last
- euchromatin replicates early
- heterochromatin replicates late
- homologous recombination
Autoradiography
image of radioactive particles as they degrade
Pulse-Chase experiment
pulse = cells are exposed to a labeled (ex: radioactive) compound
chase = cells are in normal version of that compound
find more resources at oneclass.com
find more resources at oneclass.com
Endocycle
replication of DNA without mitosis or meiosis
OriC
(in prokaryotic replication)
- DNA sequence 230bp
- consensus sequence
- 9mers = 9 base con. seq.
- 13mers = 13 base con. seq.
consensus sequence
alignment of bases or amino acids to identify conserved sequences
dnaA
(in prokaryotic replication)
- binds oriC
- changes conformation of double helix
- opens bubble at AT rich region of oriC
- recruits dnaB, dnaC
dnaB
(in prokaryotic replication)
- aka helicase
- hexamer(6 subunits)
dnaC
(in prokaryotic replication)
- chaperone
- aids in the binding of dnaB
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Starts from the beginning = doesn"t require a primer. Ex: rna synthesis can be "de novo"; dna synthesis can"t be. Squash cells and dip them in photographic emulsion. Autoradiography image of radioactive particles as they degrade. Pulse-chase experiment pulse = cells are exposed to a labeled (ex: radioactive) compound chase = cells are in normal version of that compound. Endocycle replication of dna without mitosis or meiosis. 13mers = 13 base con. seq. consensus sequence alignment of bases or amino acids to identify conserved sequences dnaa (in prokaryotic replication) Opens bubble at at rich region of oric. Recruits dnab, dnac dnab (in prokaryotic replication) Aids in the binding of dnab dnag (in prokaryotic replication) No phosphodiester bond between 3"oh and 5"po4 on adjacent okazaki fragments. Fixed by ligase and atp topoisomerase ii (in prokaryotic replication) aka gyrase. Breaks phosphodiester bonds of both strands to pass one strand through and reduce coiling tus.
