MCDB 2150 Study Guide - Midterm Guide: Southern Blot, Northern Blot, Chromatin Remodeling
2 Mar 2017
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Document Summary
Transcriptionally inactive regions of the genome are typically heavily methylated: methylation of cytosine residues is often associated with decreased transcription levels, promoters: Always upstream of the gene within 100 bases of the transcription initiation site. Position can be upstream, downstream, or within the gene. May influence the expression of more than one gene. Responsible for tissue- and time- specific gene expression. Nucleic acid blotting is widely used in recombinant dna technology. Non-coding rnas: small interfering rnas (sirnas) Post transcriptional and post translational gene regulation: example of post-transcriptional gene regulation: regulation of the stability of mrna, example of post-translational gene regulation: Activation of a protein under specific cellular conditions (ex. Addition of ubiquitin to a protein targeting it for degradation. Chromatin remodeling refers to changes in dna/chromosome structure that can influence overall gene output. Promoters are sequences in dna to which transcription factors and rna polymerase bind to initiate transcription.
