BCHM 403 Study Guide - Final Guide: Cooperative Binding, Enzyme Kinetics, Myoglobin

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plumgerbil611

15 Sep 2016

School

Binghamton University

Department

Biochemistry

Course

BCHM 403

Professor

Musselman

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EXAM TOPICS SOLMAZ

• Draw AA

• Know Polarity / Charges

• Draw Peptide Bond

• Covalent & Non-Covalent

• Electrostatic

• H-bond

• VDW

• Hydrophobicity (PO)

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• Methods for Protein Purification

o Principle for separation

▪ Assay

▪ SDS-PAGE

▪ Salting-out

▪ Dialysis

▪ Ion-Exchange Chromatography

▪ Affinity Chromatography

▪ Nickel-Affinity Chromatography

▪ Gel-Filtration

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• Cooperative binding with Hemoglobin (Hb) and Myoglobin (Mb)

o Hill Plot

• How do enzymes accelerate reaction rates?

• Michaelis Menten Kinetics

o Reaction Scheme

o Michaelis Menten Equation

o Define & How to determine experimentally

▪ Vmax

▪ Km

▪ Vo

▪ Kcat

▪ K1

▪ K-1

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BCHM 403 Study Guide - Final Guide: Cooperative Binding, Enzyme Kinetics, Myoglobin

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