PLANTBI C112 Study Guide - Quiz Guide: Primase, Dna Gyrase
23 Dec 2018
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Document Summary
2 copies of dnapol iii: adds deoxyribonucleotides. Gyrase : upstream, removes the supercoils by cutting helix and passing a strand thru. Primase : adds an rna primer to the 3" end, multiple times on lagging strand, only once on leading strand. 2 tau subunits : hold 2 dnapols and helicase together. Dnan : beta sliding clamp that holds dnapoly onto dna, also called subunit. Plasmid segregation is easier to study bc plasmids aren"t essential for viability except under imposed conditions. No segregation system - plasmid is eventually lost if selection isn"t maintained. Dedicated segregation system - plasmid maintained in pop even w/out selection. Parr : binds to dna sites called parc. Parm : an actin homolog that binds parr and polymerizes to push 2 plasmids apart, its laments are stabilized by binding to parr-parc. When parm laments are not stabilized by binding to parrc, they display dynamic instability, polymerizing a while then undergoing rapid catastrophes where laments depoly.
