CHM ENG 170A Midterm: cheme170B-sp2009-mt1-Keasling-soln
8 Jan 2019
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6 march 2009: growth models, using a monod growth model, a line was fit to e. coli growth data as shown below in. The same strain of e. coli was grown in a similar experiment using 20% more glucose. Draw your estimate on the plot below given that max was 2. Set up mass and substrate balances based on this data. Hint: the substrate concentration that yields the highest growth rate is scritical = Ks is about 30 (many things will do so long as this is shown) Ki is about 1080: protein properties and structure, a peptide has the sequence glu-his-trp-ser-gly-leu-arg-pro-gly. Some shown work will get partial credit (3 points) Answer: turns/beta sheets at residues 7 and 19 (prolines are good for turns due to their cyclic structure) disulfides at the cysteines residues 13 and 24. (5 points: many proteins catalyze biochemical reactions. They bind to substrates, but are never covalently attached to substrate or product.
