CHM ENG 170A Study Guide - Midterm Guide: Biochemical Engineering, Corynebacterium Glutamicum, Histidine
8 Jan 2019
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Name: __________________________________________________ sid: ___________________________________ Problem 1: ________ pts/20 pts. Problem 2: ________ pts/20 pts. Problem 3: ________ pts/20 pts. Problem 4: ________ pts/20 pts. Problem 5: ________ pts/20 pts. Total: ________ pts/100 pts. Enzymatic conversion of two substrates (20 pts) Suppose that two substrates are required for an enzymatic conversion according to the following mechanism: E + s1 es1 k1. 2/12: histadine and aspartic acid are important amino acids in this reaction. What is the total charge of the two amino acids at ph 5. 8 where the reaction takes place? (5 pts) The kinetic model of lactose hydrolysis by aspergillus niger lactase can be described as follows (scott et al. , 1985): Two enzymes are immobilized on the same nonporous beads. For enzyme a, the substrate is s1. For enzyme b, the substrate is s2. The product of the first reaction is s2. The relevant parameters of the reaction are given below:
