BCH 361 Study Guide - Initiator Element, Coiled Coil, Adenine
Document Summary
Insensitive to a-amanitin ribosomal initiatior element (rinr), just upstream of initation site: upstream promoter element (upe), ~150 bp upstream, rna poly ii: makes mrna. Controlled by wide array of promoters: rna poly iii: makes trnas, 5s rna, and some small rnas. Gc box common in continuously expressed genes. Caat, gc boxes can be on either strand of dna: enhancers: cis acting elements with no promoter activity stimulates effectiveness of promoters even when located thousands of nts from start site. Operate in conjunction with specific enhancer-binding proteins: has carboxy-terminal domain (ctd) of highly conserved repeats; interact with many. Tfs and regulates transcription: rna polymerase ii assembly, in addition to tfiis, other proteins can stimulate or repress the transcription of specific, mediator: large complex that acts as bridge between enhance-bound activators and proteins at the promoter. Does not interact with the dna, just bridges gap between tfs and proteins. Arg + c-g: protein binding motifs.
