Biology 2581B Study Guide - Midterm Guide: Genome Size, Parenchyma, Contig

• School: Western University
• Department: Biology
• Course: Biology 2581B
• Professor: David R Smith

Document Summary

Units: length - base pairs (bp) or mass - grams (g: base pairs. 1,000,000,000 = 1 gb: nt (nucleotide) vs bp. Haploid human nuclear genome: mass: ~3 picograms length: ~3 billion bp (3 gb) Human mtco genome: ~ 16kb and ~0. 0000016 pg. What about other cells: hepatocyte cell of the main parenchymal tissue of the liver, erythrocytes. Estimating genome size sequencing and assemble today"s te(cid:272)h from the organism (ex. polytomella) You get the data send to commercial sequencing (e. g. illumina) they have millions of sequencing reads sequencing reads: 100s bp long. Overlap the reads to assemble them to a big piece: genome size = combined length of different contigs (= overlapping sequence data; or reads) Commercial sequencing: not great for long nuclear genomes. (cid:449)e don"t get the perfe(cid:272)t pi(cid:272)tures (cid:271)e(cid:272)ause there are gaps between contigs. Would get excessively high number of combined contigs bc they are not combined completely due to the caps!!!!