Biology 2382B Study Guide - Midterm Guide: Schizosaccharomyces Pombe, Cyclin-Dependent Kinase 1, G2 Phase
19 Aug 2017
School
Department
Course
Professor
Document Summary
Control of g2 m transition in s. pombe: Loss of cdc2 activity (recessive) prevents s. pombe from entering m phase. You do not have fission forming, and the cell will get bigger and bigger. The cell does not divide, but there is growth. The kinase activity is lost: gain of cdc2 activity (dominant) brings on mitosis earlier. The kinase is always on, so it keeps on telling the cell to divide. You get small cells because there is no growth: cdc2 is transcribed and translated throughout cell cycle and is also a cyclin dependent kinase (cdk) Cdc2 in pombe is cdc28 in cerevisiae: cerevisiae: cdc28, pombe: cdc2, human versions of yeast cdks exist, which can functionally complement. There are four cdks in humans, but there is one that is identical to the yeast versions. You can take the that one and put it in yeast to rescue the mutations.
