Biochemistry 2280A Study Guide - Final Guide: Northern Blot, Agarose Gel Electrophoresis, Southern Blot
2 Dec 2015
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Extrinsic factors that affect the tm (outside the molecule) What does adding salt to the solution do to the tm of a dna. Extrinsic factors that affect the tm (outside the molevule) Solvents that disrupt the hydrogen bonding, interfere with base pairing and lowers the tm. Melting is reversible: called renaturation, hybridization or annealing, hybridization requires complementary strands. Hybridization will detect complementary nucleic acid sequences: hybridization are often done after transferring the nucleic acid to a membrane, southern blot: dna to dna on the membrane, northern blot: dha to rna on the membrane. Microarrays: dna to dna on glass slides (instead of nitrocellulose: used for profiling rna/cdna in different cell types mrna cant be cloned we have to convert it to cdna using reverse transcriptase. Cdna is complementary dna copy of the mrna. Polymerase chain reaction (pcr: can amplify a lot of the dna of interest from a pool of other.
