Anatomy and Cell Biology 3309 Study Guide - Midterm Guide: Transmission Electron Microscopy, Haematoxylin, Eosin
22 May 2018
School
Department
Professor
Histology 3309
LAB 1
Tissue compositions
Made of 4 main components
- Carbohydrates
- Nucleic acids
- Proteins
- Lipids
- Use a variety of stains to highlight these components
Hematoxylin and Eosin (H&E)
- Hematoxylin
o Basic dye
o Has a +ive charge
o Has high affinity for –ively charged components
o RNA, DNA, ribosomes, nucleic acids etc
o Stains blue
o Basophilia = when something –ive takes on a
basic dye
- Eosin
o Stains pink
o Has a –ive charge
o Acidic dye
o High affinity for +ively charged components
- When the 2 stains are combined, we can highlight the nucleus, nucleolus, proteins etc
Periodic acid-schiff (PAS)
- Loves carbohydrates (sugars)
- Stains deep magenta/pink color
- Stains glucose, mucus, glycogen, basement membranes etc
Osmium Tetroxide
- Stains lipids (fatty components)
- Stains black
How can we view these cells/tissues?
- Light microscopy
o Resolution – 0.2um (200nm)
o Identify nuclei, nucleoli, cell boundary, cytoplasmic components
- Transmission Electron Microscope
o Resolution = 3nm
o Identify Plasma cells, mast cells
o Used to analyze the ultrastructure of cell (identify golgi, ribosomes)
o Always in grey scale
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