MGY277H1 Study Guide - Final Guide: Antonie Van Leeuwenhoek, Agostino Bassi, Louis Pasteur
14 Dec 2018
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Document Summary
In dark-field microscopes light is direct towards specimen at an angle and only light scattered by specimen enters objective: cells stand our as bright against a dark background. In electron microscopes they use em lenses, electrons, and fluorescent screen replace glass lenses, visible light, and eye. Of mycolic acid - can"t use in mycobacteria: simple staining: involves one day (e. g. increase contrast when viewing specimen under light microscope, differential staining: distinguish different types of bacteria (e. g. gram stain) Fluorescence microscopy: some fluorescent dyes bind to compounds found in all cells (e. g. etbromine for dna) and some bind to structures found on microbes, and some are changed by cellular processes (e. g. living vs dead cells) Immunofluorescence is a technique used to tag specific proteins with a fluorescent compound. So does not require energy: active transport: movement against a conc. Gradient requires energy either through: atp or proton motive: rarely used by prokaryotes force commonly used by bacteria.
