CSB349H1 Study Guide - Comprehensive Final Exam Guide - Gene, Dna, Messenger Rna
20 Nov 2018
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Probes are hybridized to fixed cells or tissues. Also works if we denature chromosomes and add a large amount of the. Dna probe which will eventually base-pair with the complementary denatured chromosome: this is followed by washing step, to wash off the probes that are not hybridized to the chromosome and then observed under a fluorescent microscope. # of fluorescent tags = # of copies. Transposable elements (ex. of interspersed repeats): dna transposons, cut themselves out of the genome and re-insert themselves in another region. Dna transposons: transcribe dna into rna (rna intermediate), which is later reverse transcribed back into dna. Retrotransposons are very similar to rna retroviruses: Dna retrotransposons ltr (long terminal repeats: rna produced is not packaged & released; instead the cdna is integrated in genome. Long interspersed elements mechanism of transposition: lines are non-ltr elements, lines are highly abundant elements in our genome, replicate themselves and position themselves inside our genome; are known to being.
