CLAA05H3 Study Guide - Final Guide: Translocon, Cisterna, Radionuclide
9 Feb 2015
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Cultured cells can be obtained in large quantity. Most cultures contain only a single type of cell. Wide variety of different cells can be grown in culture http://www. fed. cuhk. edu. hk/~johnson/teaching/cell_division/cell_division. htm. Cultured cells respond to treatment with drugs, hormones, growth factors & other active substances. To treat: juvenile diabetes, alzheimer"s, parkinson"s, spinal cord injuries, etc. H&e (stain) of sections from gene knock-out mouse. For small, unstained specimens like living cells. Useful in examination of intracellular components in cells (fixed or living) at relatively good resolution. Resolution: the extent to which fine detail in specimen can be discriminated or resolved. Depends on the wavelength of light eg. dynamic motility of mitochondria, mitotic chromosomes. Image of scattered electrons collected on photographic emulsions eg. for ultrastructure analysis. Scanning electron microscopy (sem: used to view surfaces of cells, tissues and whole animals. After drying, specimen is coated with carbon & metals. Gives images their 3-d quality & allows visualization of outer cell surface.
