BIOB11H3 Study Guide - Telomere, Globin, Hybridization Probe
15 Mar 2013
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Document Summary
Deoxyribonucleic acid (dna) is a molecule encoding the genetic instructions used in the development and functioning of all known living organisms and many viruses. Dna is made of a double stranded, antiparallel helix. Complementary base pairing occurs by hydrogen bonding in which a-t and c-g bond with one another. Early work on dna which uses the physiochemical approaches include: dna is a polynucleotide chain, estimates made of genome size, values were tiny yet enormous . Genome: approxiamately 3. 2 billion base pairs: base pairing rules established by analyzing base composition, dna absorbs light in ultraviolet range, renaturation experiments defined complexity. [a] + [t] doesn"t = [g] + [c: this is because a t bonds are held by 2 hydrogen bonds, c g bonds are held by 3 hydrogen bonds. It works by recording the ring structures of dna which absorb in the ultraviolet range. Dna absorptions maximum range is approximately 260 nm. Absorbance can be used to determine dna concentration.
