UTM102H5 Study Guide - Final Guide: Dependent And Independent Variables

Design an experimentin which you will test the effect of an acidic fluid on enzymaticactivity. (Recall: enzymes are proteins.) To complete this project,it may be useful for you to review the Scientific Method Tutorial (found the CourseContent section of the classroom under the Science Learning Centerlink) and the Scientific Method lab (Lab 1), so that you canbetter understand how to design an experiment. It may also behelpful for you to review Lab 4 (Enzymes). As you review Lab 4, youwill be reminded that there are several factors that impactenzymatic activity: pH, temperature, and amount of reagent. Feelfree to refer to observations and information from Lab 4 as youcomplete the questions required in the Final Applied Project (seethe questions below).

As you design yourexperiment for this project, please remember that you are trying toexamine how an acidic fluid will modify the outcome of an enzymaticreaction. To successfully complete this project, you will need toidentify the question(s) being asked in your experiment and thehypothesis that you are testing. In your experimental design, youmust clearly explain what you are doing. That means that you willneed to identify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must alsothoroughly explain how the addition of the acidic fluid impactedthe overall reaction process (this information will help you toanswer question 4).

Hint: Keepin mind that the acid will change the environmental conditions ofthe experiment (for example, a low pH value could change the shapeof the active site on the enzyme protein), without directlyparticipating in the reaction.

please address the following:

1. What question are you asking?Clearly state your hypothesis.

2. Design an experiment. Provide adetailed account of the materials and methods used to conduct theexperiment. Also include the methods for data collection andanalysis.

3. Conduct the experiment and recordyour results below. What did you observe? Which samples showedbubbling?

4. Use your knowledge about factorsthat effect enzymatic activity to interpret your results. It may benecessary for you to refer to your lab manual and/or otherresources. What effect did the acid have on the enzyme?

5. Was your hypothesis supported? Whatis your conclusion? Explain your answer.

6. Cite all references used tocomplete your Final Applied Project.

Design an experiment in which you will test theeffect of an acidic fluid on enzymatic activity. (Recall: enzymesare proteins.) To complete this project, it may be useful for youto review theScientific Method Tutorial (found theCourse Content section of the classroom under the Science LearningCenter link) and the Scientific Method lab (Lab 1), so thatyou can better understand how to design anexperiment. It may also be helpful for you to review Lab4 (Enzymes). As you review Lab 4, you will be remindedthat there are several factors that impact enzymatic activity: pH,temperature, and amount of reagent. Feel free to referto observations and information from Lab 4 as you complete thequestions required in the Final Applied Project (see the questionsbelow).

As you design your experiment for this project,please remember that you are trying to examine how an acidic fluidwill modify the outcome of an enzymatic reaction. Tosuccessfully complete this project, you will need to identify thequestion(s) being asked in your experiment and the hypothesis thatyou are testing. In your experimental design, you mustclearly explain what you are doing. That means that you will needto identify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must also thoroughly explain how the addition ofthe acidic fluid impacted the overall reaction process (thisinformation will help you to answer question 4).

Hint: Keep in mind that the acid will changethe environmental conditions of the experiment (for example, a lowpH value could change the shape of the active site on the enzymeprotein), without directly participating in thereaction.

Lab Materials

You may need the following, depending on yourexperimental design.

Materials in your lab kit:

• none

Additional materials you may need:

• plastic beakers or cups
• hydrogen peroxide solution (from Lab4)
• yeast (from Lab 4)
• sample of fresh meat, about 1 cm cubed insize (unprocessed and uncooked, e.g., liver, steak, fish, orpoultry%u2014particularly organs)
• sample of fresh vegetable, about 1 cmcubed in size (e.g., potatoes or other root vegetables)
• a pen for labeling the beakers orcups
• a ruler with centimeter markings

Remember, the goal of this project is for you toevaluate the effect of an acid on enzymatic activity. Aspart of this assignment, you must identify your source of enzymeand the acid used. As you work though this project, youmust also make sure that the following questions/points areaddressed:

1. What question are you asking? Clearly state yourhypothesis.

2. Design an experiment. Provide a detailed account of thematerials and methods used to conduct the experiment. Also includethe methods for data collection and analysis.

3. Conduct the experiment and record your results below. Whatdid you observe? Which samples showed bubbling?

4. Use your knowledge about factors that effect enzymaticactivity to interpret your results. It may be necessary for you torefer to your lab manual and/or other resources. What effect didthe acid have on the enzyme?

5. Was your hypothesis supported? What is yourconclusion? Explain your answer.

6. Cite all references used to complete your Final AppliedProject.

Design an experiment in which you will test theeffect of an acidic fluid on enzymatic activity. (Recall: enzymesare proteins.) To complete this project, it may be useful for youto review the Scientific Method Tutorial (foundthe Course Content section of the classroom under the ScienceLearning Center link) and the Scientific Method lab (Lab 1),so that you can better understand how to design an experiment. Itmay also be helpful for you to review Lab 4 (Enzymes). As youreview Lab 4, you will be reminded that there are several factorsthat impact enzymatic activity: pH, temperature, and amount ofreagent. Feel free to refer to observations and information fromLab 4 as you complete the questions required in the Final AppliedProject (see the questions below).

As you design your experiment for this project,please remember that you are trying to examine how an acidic fluidwill modify the outcome of an enzymatic reaction. To successfullycomplete this project, you will need to identify the question(s)being asked in your experiment and the hypothesis that you aretesting. In your experimental design, youmust clearly explain what you are doing. That means that you willneed to identify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must also thoroughlyexplain how the addition of the acidic fluid impacted the overallreaction process (this information will help you to answer question4).

Hint: Keep in mind that the acid will changethe environmental conditions of the experiment (for example, a lowpH value could change the shape of the active site on the enzymeprotein), without directly participating in thereaction.

please address the following:

1. What question are you asking? Clearly state yourhypothesis.

2. Design an experiment. Provide a detailed accountof the materials and methods used to conduct the experiment. Alsoinclude the methods for data collection and analysis.

3. Conduct the experiment and record your resultsbelow. What did you observe? Which samples showed bubbling?

4. Use your knowledge about factors that effectenzymatic activity to interpret your results. It may be necessaryfor you to refer to your lab manual and/or other resources. Whateffect did the acid have on the enzyme?

5. Was your hypothesis supported? What is yourconclusion? Explain your answer.

6. Cite all references used to complete your FinalApplied Project.