CHM311H5 Study Guide - Final Guide: Intersystem Crossing, Photodiode, Electron Avalanche
Document Summary
Involves processes that provide/enhance a fluorophore in the excited electronic state to return to a ground electronic state. Static (pre-association: formation of a bound complex between fluorophore and quencher that does not fluoresce, has similar relationship to stern-volmer relationship. Transfer rates drop off dramatically with distance ket = kj e-2rda/l. Absorption energy of photon promotes an electron from ground state mo to an excited state m. o. Fluorescence excited mo relaxes to ground state mo releasing energy as photon. Electronic transitions between atomic orbitals (ao) also occur. Atomic absorption energy of photon promotes an electron from ground state ao to an excited state ao. Atomic emission excited ao relaxes to ground state ao releasing energy as photon. However, to measure these molecules, first break them down into their constituent molecules. Advantages of aa: most atoms are in ground state, therefore, providing more transitions to investigate. Flame source are not sufficient enough to provide enough electron excitation and emission providing low background.
