BIOL 112 Study Guide - Midterm Guide: Nucleosome, Telomere, Guanine
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BIOL 112 Full Course Notes
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Purine base (a/g) with pyrimidine same distance between bases in the regular hydrogen bonding pattern bind to active site. Non- bind to a different site rate and changes shape. Irreversible bind to active site. base pairs have same base (t/c) two the strands. stacking interactions between bases above and below: same, same. Dna template, primers, taq dna polymerase, buffers initially the strands of dna are longer since the dna polymerase keeps going until there is no more dna or falls off. By the 5th cycle, you are amplifying only (almost) the target region since it shortens each round. Denaturation, annealing (solution cooled, primers anneal to their complementary sequence), extension (dna pol extends). Polymerization: incoming nucleotides accepted if correctly base pair. 3" oh of growing strand attacks high energy phosphate bond of incoming nucleotide providing energy. *dntp stands for deoxynucleoside triphosphate (datp, dttp, dctp, dgtp) Telomerase enzyme: contains rna template that elongates template via telomere repeats.
