BIOL370 Study Guide - Hsp90, Hsp70, Northern Blot

60 views9 pages

azureoyster67

4 Feb 2014

School

University of Waterloo

Department

Biology

Course

BIOL370

Professor

Dinu Nesan

For unlimited access to Study Guides, a Grade+ subscription is required.

Document Summary

Median lethal time: the period of time required for 50% of a large group of organisms to die following a specific dose of an injurious agent, such as a drug or radiation. The 3rd experiment that was performed was a test of survival where they measured and compared the median lethal times at various temperatures for adult and larvae. The median lethal time: period of time required for 50% of the organisms to die due to the heat stress. As you can see, the general trend was that mortality rate increased as the temperature increased for both larvae and adult midges. However, what they found was that from 4-25 c, larvae had a greater survival rate than adults, but once it reached 30 c, the adult midges had greater survival than the larvae. These graphs are just showing the same general trend as the table from the previous slide.

Related Questions

Question 5. Dr. Campbell used an elliptical trainer for 40 minutes at the gym. Although the workload remained constant, Dr. Campbellâs heart-rate rose to 150 beats per minute during the first 5 minutes, stayed at that level for the next 25 minutes and then started to rise. It was 180 beats per minute at the end of the exercise period.

What was the time interval between QRS complexes when Dr. Campbellâs heart rate was 180 beats per minute?

a. 3.3 ms

b. 33 ms

c. 333 ms

d. 3333 ms

Question 6. In the question 5 above, Dr. Campbellâs heart rate is most likely to have risen during the last 10 minutes of exercise because his:

a. lungs were becoming congested

b. atrial cells were fatigued

c. extracellular Ca2+ concentration had increased

d. hematocrit increased due to sweating

Question 7. A Physiology student is sitting still in a cold igloo. After 30 minutes, she stands up, walks slowly around the igloo, and suddenly starts to shiver.

The student preserved their core temperature while they were sitting by:

a. depressing their Frank-Starling mechanism

b. decreasing the extracellular Ca2+ concentration

c. relaxing smooth muscles in their peripheral circulation

d. increasing the resistance of arterioles in their arms and legs

Question 8. In the question 7 above, the student started to shiver soon after walking because:

a. their atria started to fibrillate

b. their ventricles started to fibrillate

c. blood moved to the periphery, became cold, and chilled the core when it returned to the chest

d. the ATP concentration in their skeletal muscles had dropped to zero

Please use the following scenario for answering the questions 9 and 10.

A newly discovered drug binds to myosin molecules in cardiac muscle (and nowhere else) and increases the probability that myosin molecules in ventricular cells will bind to actin filaments when the intracellular Ca2+ concentration rises. The drug is being tested as a potential therapy for patients with heart failure.

Question 9. This drug might help people who have low cardiac output by:

a. increasing peripheral resistance

b. increasing the force developed by ventricular cells

c. increasing the isovolumic contraction time

d. increasing heart rate

Question 10. The increased binding of cardiac myosin molecules to actin might prevent the drug from being a useful treatment by:

a. slowing ventricular relaxation

b. decreasing the duration of systole

c. decreasing resistance of capillaries

d. slowing heart rate

cyanduck846

What are symptoms indicative of an infection by Pseudomonas aeroginosa? Can you extrapolate the symptoms found in this case report that indicate infection by Pseudomonas aeroginosa?

A 45-year-old man was referred to our hospital with a 3 day history of productive cough and chest pain. He denied any dust exposure, illicit drug use, or contact with sick people. He had been administered with oral antihyperglycemic agents to treat controllable type 2 diabetes mellitus for over 15 years. His vital signs were as follows: blood pressure, 104/66 mmHg; heart rate, 128 beats min; respiratory rate, 24 min; body temperature, 102F; and 98 % oxygen saturation with ambient air, suggesting systemic inflammatory response syndrome (SIRS). Physical findings were normal except for decreased respiratory sounds right upper lung. A chest X-ray showed a homogeneous infiltrate in the right upper lobe, indicative of penuomonia. Thoracic CT showed consolidation in the right upper lobe together.

His serum laboratory findings and white blood cell counts were normal. Sputum samples were taken for culture, and revealed the presence of significant neutrophil presence. The patient was immediately treated him with ampicillin, but he went into shock 7 hours later and required aggressive treatment including intubation. In addition, increased serum procalcitonin and endotoxin were found, suggesting that septic shock. At that time, the treatment was immediately changed to doripenem. On day 3, his general status rapidly deteriorated and he died of septic shock.

Question 1. Increased peripheral edema could be caused by:

a. blocked lymph vessels

b. decreased capillary blood pressure

c. increased concentration of albumin in the blood

d. slowed heart rate

Question 2. The pulse pressure of a healthy student is most likely to be:

a. 5 mm Hg

b. 30 mm Hg

c. 95 mm Hg

d. 120 mm Hg

Question 3. beta-blockers inhibit the activity of beta-adrenergic receptors and change the function of the heart by:

a. increasing heart rate and increasing contractility

b. increasing heart rate and decreasing contractility

c. decreasing heart rate and increasing contractility

d. decreasing heart rate and decreasing contractility

Question 4. In cardiac pacemaker cells, the L-type calcium channels:

a. open during diastole

b. close when the membrane potential rises above threshold

c. are located in the cell membrane

d. pump Ca2+ ions into the sarcoplasmic reticulum