BIOL241 Study Guide - Midterm Guide: Microbiological Culture, Exotoxin, Pharynx

• School: University of Waterloo
• Department: Biology
• Course: BIOL241
• Professor: Barbara Butler

Document Summary

Scale up: o going from a very small scale reaction that we are used to in the lab (test tubes) up to an industrial sized process (hundreds of thousands of litres) = scale up o involves step wise process, lots of biomass, low oxygen solubility in large volume of liquid, oxygen transfer has to change as you scale up o oxygen transfer difficult to obtain in large scale o mixing and aeration easily accomplished in small volumes o temp control must change with scale up o sterilization protocols must change with scale up o bioengineers skilled in gas transfer, fluid dynamics, mixing, thermodynamics. o continuous flow type of reactors (cid:224) quite often get a lot of foam getting produced, put in antifoam to reduce (tubes going to sides are trying to collect the foam) o huge bioreactors in b, go up a few stories.