BIOL240 Study Guide - X-Ray Crystallography, Reverse Transcriptase, Saccharomyces Cerevisiae
9 Dec 2013
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Genomics: discipline involving mapping, sequencing, analyzing and comparing genomes. Functional genomics: assigning function to unknown genes by analyzing biochemical and physiological. Proteomics: analysis of the structure, function and regulation of proteins. Transcriptomics: analysis of the expression of all of the transcripts in the genome at once. Metagenomics: extraction and analysis of dna directly from an environmental sample. Automated/cycle-sequencing methods: larger dna fragments undergo larger electrophoretic. Shotgun sequencing: sequencing in one step ends randomly, shearing dna into short pieces, cloning dna in plasmid, and sequencing those fragments at their, software aligns/assembles sequences - nds overlapping sequences. Biol 240 fall 2013 transcriptase: mrna is then converted into complementary dna, or cdna, using the enzyme reverse, dna fragments then ligated into a cloning vector, select fragment of interest (based on resistance to antibiotics, etc) complementary sequence. Transcriptomics transcriptome: collection of transcribed mrna molecules in a cell (transcripts) Southern blot: detecting speci c dna sequences by hybridization using labeled probes of.
