BIOM 4070 Study Guide - Quiz Guide: Red Blood Cell, Goblet Cell, Hemoglobin
28 Sep 2016
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Doesn"t differentiate carbohydrates, it will blend with cytoplasm. Pink: basic things (+ive), acidophilic/eosinophillic, mitochondria (lots of transmembrane proteins), collagen, cytoskeleton. Purple: acidic things (-ive), basophilic, nucleic acid (dna & rna), rer (lots of ribosomes, outweighs the protein) Indicates relative protein levels (darker blue = more protein) Based on density (dense areas appear dark because more electrons reflect back) Higher resolution than lm because it has a shorter wavelength than light and therefore higher energy/frequency, and can cut thinner slices. Uses lighter metals (like gold), scatters reflected electrons. Nuclei are at basal end and proteins (digestive enzymes are stored in zymogen vesicles, stain purple in h&e or dark in em) are at apical end (about to be secreted). Nuclei: may appear heterogenic (purple because of nucleic acid but pink because of proteins like histones) but overall tend to be mostly purple in h&e, dark in em, light blue in toluene blue (less relative protein).
