BIOC 3570 Study Guide - Final Guide: Gene Expression, Lipid Raft, Growth Factor

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 3570
• Professor: litchfield

Document Summary

Discovery of covalent modification as a regulatory mechanism using glycogen phosphorylase: Phos b is inactive form and phos a is active form. Isolated the inactive form from the muscle which could be converted to the active form. Aged phos a extract lost atp with aging rendering it inactive. 1st example of protein phosphorylation as a regulatory mechanism. Phos b (dimer) + 2 atp using a kinase-->2 adp and phos a (dimer) Phos a+2 waters using a phosphatase-->phos b + 2 phosphates. Showed how one modification changed phos a from active form to inactive and phos b from inactive to active form. Addition of camp or ca2+ to muscle extracts lead to kinase activation and phos b conversion to active phos a. Ca2+ directly activated the kinase to coordinate glycogen degradation with muscle contraction camp required atp which led to the identification of a second kinase in the pathway.