BIOC 2580 Study Guide - Final Guide: Stem-Loop, Scissile Bond, Drosha

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 2580
• Professor: Shannon De Roo

Document Summary

Pathway to regulate gene expression in eukaryotes only. Involves sirnas or mirnas which are short single-stranded rnas between 20-30 nucleotides in length that have complementary sequence to other, larger rna molecules sirnas and mirnas with protein complex risc (rna-induced silencing complex) regulate many processes. Short rna molecules are the targeting part of the system. Slicer(ago2) is the catalytic part of the system. The kind of pairing that occurs between the rna target and sirna or mirna determines how risc proceeds sirna: extracellular source possibly from dsrna viruses or plasmids designed to knock out a gene expression. Exact match to target site so induce cleavage of target mirna: intracellular source that is transcribed by our genes. Imperfect complementarity with their target so induce translational repression. Regardless of origin, both molecules are fed into the same rnai pathway with the same enzymes, just with different outcomes (repression of that gene expression) mirna biogenesis.