GENET270 Study Guide - Midterm Guide: Polysaccharide, Helicase, Topoisomerase
18 Dec 2017
School
Department
Course
Professor
Document Summary
Rod shaped or spherical: coli model organism and pathogen, coelicolor produce antibiotics. Gm- (pink) or gm+ (purple) depending on structure (negative has thinner peptidoglycan, porins and periplasm and lipopolysaccharides that make it impermeable) Prototrophs grow on minimal media, auxotrophs require organic substances other than carbon source for growth. Can carry foreign dna to new hosts, and therefore are responsible for some bacterial evolution. Delivery of nucleic acid to inside of bacterium. Conversion of bacterium into phage factory to produce more. Nucleic acids within a protein coat or capsid. Phage that undergo only lytic growth are called virulent. Lysate collection of lysed cells and new phages. Burst size - # of phage released per bacterium. Phage dna is integrated into chromosome and is silent (prophage state bacterium harbouring it is called lysogen) Eop fraction of phage particles that can form a plaque. Isolation of phage stocks phage produce more rapidly than bacteria (1:1 ratio = all bacteria lysed)
