CELL301 Study Guide - Final Guide: Cytotoxicity, Membrane Potential, Atp Hydrolysis

• School: University of Alberta
• Department: Cell Biology
• Course: CELL301
• Professor: Richard Wozniak

Document Summary

Closing: bcl-2, bcl-xl, high adp/atp ratio, cyclosporin a. Mutation in sod1 linked to genetic als (amytrophic lateral sclerosis) Senses mt ros and induces dna damage upon apoptosis onset. Also a scavenger of ros in brain/retinal neurons (therefore loss of aif actually increases neurodegeneration, oxidative stress, and apoptosis) Part of the electron transport chain (shuttles electrons from complex iii to iv) Component of the apoptosome (7x apaf-1, 7x cyt. c, caspase-9) Adaptor protein implicated in control of oxidative stress response and animal lifespan. Results in production of ros because electrons are no longer passed on to complex iv. Ros-induced permeability transition and apoptosis ensue p66shc --> activated by oxidative stress (oxidation of cysteines) - allows it to interact with cytochrome c. Causes generation of ros and opening of ptpc --> apoptosis. Regulator of heme signalling in yeast (oxygen sensing) Polymerizes with heme in the presence of iron.