BIOL207 Study Guide - Woolly Mammoth, Ancient Dna, Missense Mutation

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Question 1 even under the best possible conditions dna can not last more than 100. Dinosaurs went extinct 65 000 000 years ago. However, pcr is sensitive enough to amplify the tiny amounts of intact dna remaining in less ancient fossils. The sequences obtained were compared to the sequences in living creatures to determine how closely related the species are. A lot of this research on ancient dna is being done by svante p bo. Question 2a) the left primer would have been 5"-atacg-3" and the right primer would have been 5"-gtgtg-3" . (primers are typically written 5" --- 3" because that"s how they are ordered from idt. ) Question 2b) the primer binds to the bottom dna strand and allows dna synthesis towards the right: Note that the "g" nucleotides are shown with black ink as the true yellow colour is hard to see.

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