MBB 222 Study Guide - Final Guide: Holliday Junction, Branch Migration, Recombination-Activating Gene

• School: Simon Fraser University
• Department: Molec Biol & Biochem
• Course: MBB 222
• Professor: Edgar Young

Document Summary

Mbb222 exam vocab definitions: site-specific recombination and immunoglobulin. U7-08 first site-specific recombination (as opposed to homologous recombination) Recombination = when 2 duplexes are broken then ligated again with different combinations. Site-specific recombination = type of recombination that is limited to specific sequences. This type occurs in virtually every cell, filling specialized roles that vary greatly from one species to another. Ex. ) regulation of expression of certain genes. This type of recombination involves recombinase and a unique dna sequence where the recombinase acts (the recombination site) Consists of 2 classes of site-specific recombination systems which either rely on tyrosine or serine residues in the active site, which act as nucleophiles. In end switching, one strand is broken in each duplex, which creates an oh attacking group (if considering tyr-dependent recombination) Transesterification accomplishes the switch for 2 of the 4 strands and makes the holliday junction.