MICR 221 Study Guide - Final Guide: Flaviviridae, Denitrification, Antimicrobial Resistance
24 Apr 2018
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Metazoans + uni euk by repro isolation. Horizontal gene transfer: via transformation, transposable elements, bacteriophages det by gc content or genome seq create web of life- ford doolittle, ex. E coli 18% of genes are from other species. Divergence via mutation and ns- molecular clocks assume constant mutation rate + gen time. Methods of metagenomics: culture isolate seq (problem=many unculturable, phenotype analysis: microscopes, gram stains, shot gun close- pcr w universal primers (16s rrna) ligate to vector genbank or. Rick stevens: seq earth microbiome to compare to space. 16s rrna: in every organism, stem region conserved. Van niel: everything can be used as c source for microbes, microbes found everywhere (co- dependent) Microbial loop: om is recycled not lost ocean food web (yields om, co2 and e): external e captured by microbes, microbe inter- dependence. Carbon cycle- c is moving from tundra to water as doc, dic, poc.
