BIOL 330 Study Guide - Final Guide: Rna Polymerase Ii, Rna Polymerase I, Northern Blot

Please provide correct answer and explanation to the answer,Thank you!

1. If an enterprising graduate student decided to repeat theHershey-Chase

   experiment but neglected to perform the blending portion, wherewill the

   isotopes be found?

   a. 35S will be found in the supernatant and 32P will be found inthe pellet

   b. 32P will be found in the supernatant and 35S will be found inthe pellet

   c. Both 32P and 35S will be found in the supernatant

   d. Both 32P and 35S will be found in the pellet

   e. None of the above are correct

2. Chargaff�s rules include:

   a. The ratio of A�s + T�s to G�s + C�s is always consistent

   b. A=G and T=C

   c. Purines/Pyrimidines = 1

   d. More than one of the above are true

   e. None of the above are true

3. Sea Urchin DNA contains 18% guanine (which is true); how muchthymine

   does it contain?

   a. 9%

   b. 18%

   c. 32%

   d. 36%

   e. 64%

4. E. coli DNA contains a little over 25% guanine. If samples weretaken of E.

   coli DNA and Sea Urchin DNA and denatured in water baths, whichwould

   melt at a lower temperature?

   a. E. coli DNA

   b. Sea Urchin DNA

   c. They would melt at the same temperature

   d. There is not enough information to determine the answer

   e. DNA doesn�t �melt,� it isn�t ice cream for goodness sake!

5. If testing the second generation results in tube B, which modelcan we

   conclude is correct?

   a. Dispersive

   b. Semi-conservative

   c. Conservative

   d. Semi-dispersive

   e. We can�t conclude yet, it could still be more than onepossibility

6. Phosphorus is required to synthesize dNTPs used in DNAreplication. Imagine

   you grow E. coli in media containing non-radioactive phosphorusfor many

   generations, then switch the E. coli to media containingradioactive

   phosphorous (32P). You then sample the E. coli after one roundof replication

   for radioactivity. Where will you find radioactive phosphorus inthe DNA?

   a. Both strands will contain 32P

   b. One strand will contain 32P

   c. Neither strand will contain 32P

   d. Drats! I knew I should have done suggested problem 12.19!

7. Which of the following statements about chromosomes are alwaystrue?

   I. Non-histone proteins make up about 5% of a eukaryotic

   chromosome by weight

   II. Histones have a negative charge

   III. Prokaryotes have a single linear chromosome

   a. Only Statement I is true

   b. Only Statement II is true

   c. Only Statement III is true

   d. More than one of the above statements are true

   e. None of the above statements are true

8. What portion of the human genome is made up of highly repetitiveDNA?

   a. Less than 5%

   b. 6 � 20%

   c. 21 � 30%

   d. 31 � 40%

   e. Over 40%

9. Which of the following statements are always true?

   I. Histones are very conserved between species

   II. Archaea have nuclei

   III. Prokaryotes are haploid and eukaryotes are diploid

   a. Only Statement I is true

   b. Only Statement II is true

   c. Only Statement III is true

   d. More than one of the above statements are true

   e. None of the above statements are true

10. An individual�s phenotype is a function of:

    a. The alleles they carry

    b. The environment they live in

    c. The interaction between an individuals genes and theenvironment

    d. Two of the above are correct

    e. All of the above are correct

11. A homologous pair of chromosomes showing a

    crossover are shown to the right for reference. Are all

    the sperm from one human male genetically identical?

    a. Yes

    b. No, variation occurs during mitosis

    c. No, variation occurs during meiosis I

    d. No, variation occurs during meiosis II

    e. No, variation occurs during both meiosis I and II

12. Production of gametes in humans requires which kind ofdivision?

    a. Meiotic and is reductional

    b. Mitotic and is reductional

    c. Meiotic and is equational

    d. Mitotic and is equational

    e. None of the above