LIFESCI 2L03 Study Guide - Quiz Guide: Health Care In Canada, Belleville, Ontario, Imagej
28 Apr 2018
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PART 3A: IMAGE ANALYSIS -MEASURE TRABECULAR THICKNESS.
Measure the thickness of the trabeculae in your images collected in Part 2A.
To calculate thickness, determine the midpoint of 10 randomly selected
trabeculae and use the measurement tool in ImageJ to determine the
thickness. Enter this information in Table I. Refer back to Figure 4 to see how
to measure the thickness of a trabecula (red line).
PART 3B: IMAGE ANALYSIS.
CALCULATE MEAN OSTEON AREA.
Randomly select 10 to 20 primary osteons in the images that you captured in
Part 2B. Using the ImageJ measurement tool, calculate the osteon area for 10
to 20 osteons and compute the mean osteon area for your data set. Refer
back to the information on using ImageJ from the Histology lab. You will need
to outline the
Take steps early on to be preventative -diet, exercise
$1600/month -TYMLOS -drug *research for paper
Osteoporosis is linked to factors such as lifestyle choices, genetics, age and
gender
-individuals who do not exercise have a higher risk of developing
osteoporosis
Exercising Role
-the activity of osteoblast cells goes up
○Role in creating bone
○Inactivity plays a role in bone loss
§while resistance training and weight bearing exercises
improve bone
Another lifestyle factor is diet and nutrition
-A diet lacks calcium and vitamin D
○weaker, more fragile
○bones prone to fractures
Reduction in sex hormones such as estrogen, estradiol and testosterone
-risk factor in development of osteoporosis
Bones&-Lab&Notes
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Document Summary
billion cost for canadian health care system. Cortical bone - outside (hard), spongey (trabecular bone) - inside. Look @ thin tissue samples of clavicle bone from different ages. St. thomas aglican church cemetery in belleville, ontario. 4 prepared slides of clavicle bone (different ages) Select 10 trabeculae samples and measure thickness of each. 2a: 4 slides from different ages (each is unique specimen, very valuable) Capture several regions of trabeculae on one slide (40x) A microscope with a polarizer and analyzer is set up. Capture several regions of interest for each sample (each group member. Concentric layers / lamellae surrounding a central canal (haversian) Capture four rois in which you can see the osteons. Part 3a: image analysis - measure trabecular thickness. Measure the thickness of the trabeculae in your images collected in part 2a. To calculate thickness, determine the midpoint of 10 randomly selected trabeculae and use the measurement tool in imagej to determine the thickness.
