CHEM 297 Study Guide - Quiz Guide: Resveratrol, Xining, High-Performance Liquid Chromatography
23 Feb 2016
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Cheers to antioxidants in wine analysis of trans-resveratrol in red wine. Given the transisomer of resveratrol has a maximum absorbance at the 306nm wavelength, these values are calculated at a retention time of approximately 7. 41 minutes. Overall, before the rejection of the outliers, the r2 value is 0. 998398. Since points 3 and 4 seem to be outliers, the residual plot of the corrected chart is as follows: The corrected intercept here is much closer to the ideal and theoretical (0, 0) intercept. Peak area vs ppm t-res f(x) = 160. 58x + 14. 67. Concentration of the wine sample is therefore 3. 144965 + 0. 174697 ppm a. If given an hplc with a similar setup, the concentration of each isomer in the unknown sample can be experimentally determined. Since the two isomers have a separation between the two beaks, it is possible to use a detection wavelength of.
