BIOL 301 Study Guide - Sonification, Transposable Element, Polyadenylation
6 Jan 2015
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Used for: to identify (mutated) gene responsible for gene. Expected results: linked gene = nearby low rf. Used for: to confirm candidate gene is responsible for phenotype. Other uses: to confirm fusion proteins are functional. Inserted t- dna flanked by lb, rb. Any dna between lb, rb is inserted: transposon (drosophilia, ensure endogenous promote for species is in vector. Ve: cut vector (control for self- ligation) If gene is responsible, phenotype will be rescued. Used for: screen large number of genes at once. Other uses: can compare wt to mutant. If there are more genes that are expressed in sample, probe will bind and make region brighter. Other uses: confirm other experiments (microarray, etc. ) If expression level of goi is high, you end up with more pcr product. Used for: directly measure expression level (quantitative) Other uses: identify splicing variants, determine size of full length mrna. *to quantify can choose either rt- pcr or northern hybridization.
