BIOL 200 Midterm: Principles of Illumina Solexa Sequencing

Illumina Bridge Amplification/Sequencing Illumina technology made in California can determine hundreds of bacterial identities and quantities simultaneously and easily for about $40 per sample. After the amplification of a template into a cluster by PCR, Illumina Bridge amplification products are sequenced. This is tedious and can take up to 11 days. Describe the process of “sequencing by synthesis” that Illumina uses to determine the base pair sequence of a single DNA template cluster.

In what ways is Illumina sequencing similar or different from the classic Sanger sequencing approach? Select all true statements below.

A. Both approaches use PCR

B. Illumina sequencing uses"bridge PCR" which is actually a form of isothermal amplification

C. Sanger sequencing is a "sequencing by termination" approach

D. Illumina sequencing is a "sequencing by synthesis" approach

E. Sanger sequencing uses capillary electrophoresis

F. Illumina sequencing uses a set of 6 primers for amplification