GEOL-1021EL Study Guide - Quiz Guide: Hydrophile, Particle Size, Fluorescence
8 Aug 2020
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Capillary flow action: example is paper chromatography: Column chromatography: most powerful approach where sample passes through a column containing appropriate particles (stationary phase); solvent (mobile phase) flows through the column. Cartridge format: flow can be achieved through gravity or vacuum: Gravity > 50 microns particle diameter. Vacuum < 10 microns particle diameter: smaller particle size can improve separation power; i. e. smaller particle size = higher pressure, this was moderated to high pressure lc aka hplc. It was coined by prof horvath 1970 at pittcon; it is used to generate flow for liquid chromatography; early pump pressure is only 500 psi hplc was high pressure". H was re-designated to be high" performance liquid chromatography: hplc is now one of the most powerful tools in analytical chemistry improving: It has many applications in food, cosmetics, environmental, forensic, industrial chemicals and pharmaceuticals. Uplc: ultra performance liquid chromatography, 2004 further advances in instrumentation and column tech achieved increases in:
