BIOC 3400 Study Guide - Midterm Guide: Reverse Transcriptase, Pribnow Box, Helicase

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coffeeoyster95

6 Nov 2015

School

Dalhousie University

Department

Biochem & Molecular Biology

Course

BIOC 3400

Professor

Liu Paul

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Document Summary

Dna footprinting is a method of investigating the sequence specificity of dna-binding proteins in vitro. This technique can be used to study protein-dna interactions both outside and within cells. The regulation of transcription has been studied extensively, and yet there is still much that is not known: how to find the start. Sigma factor is a part of initiation complex. Once initiation of rna transcription is complete, the sigma factor can leave the complex. Only one dna strand is transcribed (template strand); newly transcribed mrna is identical to non- template strand. Only a fraction of the genome is transcribed, while in dna replication, all of the dna is replicated. Different enzyme with different properties: transcription -- rna polymerase; dna replication -- dna polymerase ( much faster, more accurate). Slightly different base: u instead of t, ntps instead of dntps. Both extend the nucleic acid chains in 5"-3" direction. Factor: an rna-dna helicase with nucleoside triphosphatase activity ( atp dependent)

Related Questions

Which type of RNA is unique to eukaryotes?

		mRNA
		rRNA
		tRNA
		snRNA
		Its a trick, all cells have all four types of RNA.

1 points

QUESTION 2

The region of DNA to which RNA polymerase binds to initiate transcription is the:

		origin
		promoter
		enhancer
		none of the above are correct

1 points

QUESTION 3

Holoenzyme:

		is RNA polymerase core enzyme.
		is sigma factor.
		is core plus sigma factor.
		none of the above are correct.

1 points

QUESTION 4

Which of these is formed FIRST when RNA polymerase binds to a promoter?

		The closed promoter complex
		The open promoter complex
		A rho-independent terminator
		none of the above are correct.

1 points

QUESTION 5

A hairpin loop followed by a run of Us:

		is the sequence formed in the sense strand of a gene using a rho-independent terminator.
		is the sequence recognized by the rho protein.
		is the structure formed in RNA that causes rho-independent termination.
		is formed immediately after the open promoter complex is formed.

1 points

QUESTION 6

RNA polymerase:

		reads the template strand of the DNA in the 3' to 5' direction.
		synthesizes the RNA in the 5' to 3' direction.
		can begin transcription without a primer.
		all of the above are correct.

1 points

QUESTION 7

Which of the following statements is FALSE?

		Bacteria like E. coli have only one type of sigma factor.
		RNA polymerase has no proofreading activity.
		The promoter of a gene is upstream of the RNA-coding region.
		Bacterial transcriptional terminators are copied into RNA to cause termination.

1 points

QUESTION 8

If the template strand of a gene has the sequence 5' ATGCGAT 3', then:

		the RNA produced will have the sequence 5' AUGCGAU 3'.
		the RNA produced will have the sequence 5' UAGCGUA 3'.
		the RNA produced will have the sequence 5' AUCGCAU 3'.
		None of the above are correct.

1 points

QUESTION 9

The sense strand:

		is the lagging strand
		is also the template strand.
		always goes left to right.
		none of the above are correct.

1 points

QUESTION 10

Which of these is transcribed but not translated?

		mRNA
		tRNA
		rRNA
		all of the above
		tRNA and rRNA are transcribed but not translated.

please help i am a bit confused

BIOC 3400 Study Guide - Midterm Guide: Reverse Transcriptase, Pribnow Box, Helicase

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