BIOL 3P43 Midterm: Lecture 10 Condensed

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Enzymes are proteins, they catalyze reactions, anabolic/catabolic first enzyme produced industrially (1894) was the fungal amylase takadiastase as a pharmaceutical agent (for digestive disorders) Analytical uses- glucose oxidase, galactose oxidase, alcohol dehydrogenase, cholesteral oxidase. Starch processing -> liquifying it and breaking it into glucose units and the glucose isomerase turns it into fructose. Dextrose equivalents (de)- hydrolysis of glucosidic bonds leads to an exposure of reducing aldehyde groups -r-ch=o. The hydrolysis of starch to a mixture of sugar monomers and oligomers is monitored by measuring the reducing power relative to the same of pure glucose. De=100 is a fully hydrolyzed sample of starch. High-fructose corn syrup (hfcs)- hfcs has a high fructose content. Degree of isomerization (di)- the % of sugars in a syrup that is fructose. Enzymatic conversion of starch to dextrins and sugars starch-->short chain polymers (maltodextrins)-->glucose --> fructose three steps step 1- liquifaction- conversion of starch to low-de maltodextrins via alpha-amylase from bacillus.

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