BCH2022 Study Guide - Final Guide: Carbamoyl Phosphate, Citric Acid Cycle, Urea Cycle

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Urea Cycle
NH3 + CO2 + aspartate + 3 ATP + 2H2O urea (CH4N2O) + 2 ADP + 2 Pi + AMP +
PPi
Function
To eliminate excess nitrogen
Location
In the liver
Mitochondrial matrix and cytosol
Starting Point
Urea is produced from ammonia
Synthesis of carbamoyl phosphate (CP) (enzyme)
Enzyme: carbamoyl phosphate (CP)
1. Ornithine + CP Citrulline
o CP requires ATP occurs in mitochondrial matrix
2. Citrulline + Aspartate Argininosuccinate
o Occurs: mitochondria
3. Argininosuccinate Arginine + Fumarate
o Occurs: cytosol
4. Arginine Urea + Ornithine
o Ornithine: recycled back to mitochondria matrix to enable urea cycle
o Occurs in: cytosol
Know ATP is required
Connection with Citric Acid Cycle
Fumarate is an intermediate in citric acid cycle (step 7): oxidised to form
Malate
Regulation of Urea Cycle
Activity of urea cycle is regulated at the level of enzyme synthesis
Carbomoyl phosphate synthestase I is allosterically activated by N-
acetylglutamate
Balanced diet
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