🏷️ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION →
Log in
Science
13
answers
1
watching
196
views

Laboratory Automation II 

1. What does the term "sandboxing" mean with regards to implementing a LIMS? (note: this concept could be used when testing an implementing an automated assay side by side the non-automated version). How would we use a concordance study to validate this? 

 

Watch
13
answers
1
watching
196
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Avatar image
Read by 2 people
Already have an account? Log in
Share
Avatar image
Read by 2 people
Already have an account? Log in
Share
Avatar image
Read by 2 people
Already have an account? Log in
Share
Avatar image
Read by 2 people
Already have an account? Log in
Share
Avatar image
Read by 2 people
Already have an account? Log in
Share
Avatar image
Read by 2 people
Already have an account? Log in
Share
Avatar image
Read by 2 people
Already have an account? Log in
Share
Avatar image
Read by 2 people
Already have an account? Log in
Share
Avatar image
Read by 3 people
Already have an account? Log in
Share

Related questions

Please answer all 4 questions correctly.

1. Applying Concepts 

All mammals have the same size hearts relative to their body size. Do their hearts beat at the same rate?

2. Interpreting Data

a. What is the resting pulse rate of a 7,000-kilogram African bull elephant?

b. What is the resting pulse rate of a 20-gram mouse? How many complete heartbeats is that per second?

c. What general statement can be made regarding the effect of body size on heart rate in mammals?

3. Making Inferences

a. The data in the graph, plotted on logarithmic coordinates (that is, the scale rises in powers of 10), fall nicely upon a straight line. How would you expect them to look plotted on linear coordinates?

b. As you walk through the graph from left to right, the line slopes down. This is called a negative slope. What does the negative slope of the line signify?

4. Drawing Conclusions

If you plotted data for an experiment measuring body mass versus resting oxygen consumption, you would get exactly the same slope of the line as shown in this graph. What does this tell us about why body size affects heart rate in mammals as it does?

The Centers for Disease Control and Prevention and the United Nations have come together to provide a generous amount of funding to investigate the impact of natural disasters leading to environmental radiation leaks on a variety of health outcomes.  Due to your large budget, you are able to implement objective radio assays to determine levels of radiation exposure among your subjects.  What bias would the use of radio assays likely reduce the most? (Choose one best answer) 

A) Misclassification of disease

B) Diagnostic suspicion bias

C) Misclassification of exposure

D) Berkson's bias (i.e. Diagnostic Bias)

E) Hawthorne effect

Do Enzymes Physically Attach to Their Substrates?
 
When scientists first began to examine the chemical activities of organisms, no one knew that biochemical reactions were catalyzed by enzymes. The first enzyme was discovered in 1833 by French chemist Anselme Payen. He was studying how beer is made from barley: First, barley is pressed and gently heated so its starches break down into simple two-sugar units; then yeasts convert these units into ethanol. Payen found that the initial breakdown requires a chemical factor that is not alive and that does not seem to be used up during the process-a catalyst. He called this first enzyme diastase (we call it amylase today).
 
Did this catalyst operate at a distance, increasing the reaction rate all around it, much as raising the temperature of nearby molecules might do? Or did it use physical contact, actually attaching to the molecules whose reaction it catalyzed (its "substrate")?
 
The answer was discovered in 1903 by French chemist Victor Henri. He saw that the hypothesis that an enzyme physically binds to its substrate makes a clear and testable prediction: In a solution of substrate and enzyme, there must be a maximum reaction rate. When all the enzyme molecules are working full tilt, the reaction simply cannot go any faster, no matter how much more substrate you add to the solution. To test this prediction. Henri carried out the experiment whose results you see in the graph, measuring the reaction rate (V) of diastase at different substrate concentrations (S).
 
Analysis
1. Making Inferences As S increases, does V increase? If so, in what manner-steadily, or by smaller and smaller amounts? Is there a maximum reaction rate?
2. Drawing Conclusions Does this result provide support for the hypothesis that an enzyme binds physically to its substrate? Explain. If the hypothesis were incorrect, what would you expect the graph to look like?
3. Further Analysis If the smaller amounts by which V increases are strictly the result of fewer unoccupied enzymes being available at higher values of S, then the curve in Henri's experiment should show a pure exponential decline in V-mathematically, meaning a reciprocal plot (1/V versus 1/S) should be a straight line. If some other factor is also at work that reacts differently to substrate concentration, then the reciprocal plot would curve upward or downward. Fill in the reciprocal values in the table to the right, and then plot the values on the lower graph (1/S on the x axis and 1/V on the y axis). Is a reciprocal plot of Henri's data a straight line?

Related Documents

FSC239Y5
Final Exam
Study Guide
FSC239Y5 Study Guide - Midterm Guide: Combined Dna Index System, Royal Canadian Mounted Police, Polarized Light Microscopy
copperfox781
CS447 Lecture Notes - Lecture 1: Version Control, Uptodate, Unit Testing
CGSC 1001 Lecture Notes - Lecture 23: Autonomous Cruise Control System, Elon Musk, Deep Learning
apricotseal274

Weekly leaderboard

Home
Homework Help 3,900,000
Science 1,000
Start filling in the gaps now
Log in