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Biochemistry: What is the meaning of each: Km, Vmax, kcat, and catalytic efficiency?
Biochemistry: What is the meaning of each: Km, Vmax, kcat, and catalytic efficiency?
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13 Mar 2023
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Related questions
An enzyme that follows Michaelis-Menten kinetics was determined to have a Vmax of 1.0 mM sâ1, and a Km of 0.20 mM. If the concentration of enzyme that was used in the experiments that led to the above determinations was 10. nM, what is the value of the specificity constant (kcat/Km), and has this enzyme reached âcatalytic perfectionâ?
kcat/Km = 5 Ã 10â9 Mâ1 sâ1; the enzyme has not reached catalytic perfection |
kcat/Km = 50 Mâ1 sâ1; the enzyme has reached catalytic perfection |
kcat/Km = 5 Ã 10â8 Mâ1 sâ1; the enzyme has not reached catalytic perfection |
kcat/Km = 5 Ã 109 Mâ1 sâ1; the enzyme has reached catalytic perfection |
kcat/Km = 5 Ã 108 Mâ1 sâ1; the enzyme has reached catalytic perfection |
kcat/Km = 5 Mâ1 sâ1; the enzyme has not reached catalytic perfection |
kcat/Km = 50 Mâ1 sâ1; the enzyme has not reached catalytic perfection |