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13 Dec 2019
Thick-layer chromatography is performed on a preparative scale in the same general fashion as TLC but the plates have much thicker layers of silica or alumina. Relatively large amount of a mixture are spotted in a virtual band along the origin (many spots are placed side by sied). The plates are then developed in the usual fashion. Suppose a 75:25 mixture of A (Rf = 0.80) and B (Rf = 0.40) is run. Draw the developed chromatogram you should expect to see. How can A and B be recovered from the plate?
Thick-layer chromatography is performed on a preparative scale in the same general fashion as TLC but the plates have much thicker layers of silica or alumina. Relatively large amount of a mixture are spotted in a virtual band along the origin (many spots are placed side by sied). The plates are then developed in the usual fashion. Suppose a 75:25 mixture of A (Rf = 0.80) and B (Rf = 0.40) is run. Draw the developed chromatogram you should expect to see. How can A and B be recovered from the plate?
Nelly StrackeLv2
17 Dec 2019