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12 Dec 2019
The isoelectric point (pI) of a peptide is the pH at which the peptide does not migrate in an electric field. Since the peptide is zwitterionic, there are the same number of positive charges as negative charges on the peptide population. The pI can be estimated fairly accurately (within 0.1 or 0.2 pH units) from the pK values of all the proton dissociable groups in the peptide. Using pK values from the table at the right, estimate the pI value of the following hexapeptide:
Phe-Lys-Asp-Cys-Thr-Tyr
Amino Acid Side Chain pK
Arg 12.5
Asp 3.7
Cys 8.2
Glue 4.3
His 6.0
Lys 10.5
peptide NH3+ 8.0
peptide COOH 3.4
The isoelectric point (pI) of a peptide is the pH at which the peptide does not migrate in an electric field. Since the peptide is zwitterionic, there are the same number of positive charges as negative charges on the peptide population. The pI can be estimated fairly accurately (within 0.1 or 0.2 pH units) from the pK values of all the proton dissociable groups in the peptide. Using pK values from the table at the right, estimate the pI value of the following hexapeptide:
Phe-Lys-Asp-Cys-Thr-Tyr
Amino Acid Side Chain pK
Arg 12.5
Asp 3.7
Cys 8.2
Glue 4.3
His 6.0
Lys 10.5
peptide NH3+ 8.0
peptide COOH 3.4
Jean KeelingLv2
13 Dec 2019