Given: MW(kD) No. ofasp glu lys arg his
ProteinA 85.3 35 28 40 18 9
ProteinB 46.2 18 14 20 9 5
ProteinC 46.2 20 19 14 12 4
a) Assume that each of the three proteinscontains a single polypeptide chain. Estimate the number of aminoacids in each protein.
b) A mixture of the three proteins ispassed over a size exclusion column. Predict the elutionprofile.
c) A mixture of the three proteins isapplied to a cation exchange column at pH 5. Unbound protein iswashed off the column, then bound protein is eluted with a NaClgradient. Predict the elution profile, including what gets washedoff initially.
d) A mixture of the three proteins isapplied to an anion exchange column at pH 5. Unbound protein iswashed off the column, then bound protein is eluted with a NaClgradient. Predict the elution profile, including what gets washedoff initially.
Given: MW(kD) No. ofasp glu lys arg his
ProteinA 85.3 35 28 40 18 9
ProteinB 46.2 18 14 20 9 5
ProteinC 46.2 20 19 14 12 4
a) Assume that each of the three proteinscontains a single polypeptide chain. Estimate the number of aminoacids in each protein.
b) A mixture of the three proteins ispassed over a size exclusion column. Predict the elutionprofile.
c) A mixture of the three proteins isapplied to a cation exchange column at pH 5. Unbound protein iswashed off the column, then bound protein is eluted with a NaClgradient. Predict the elution profile, including what gets washedoff initially.
d) A mixture of the three proteins isapplied to an anion exchange column at pH 5. Unbound protein iswashed off the column, then bound protein is eluted with a NaClgradient. Predict the elution profile, including what gets washedoff initially.
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